Review of the concept of Profilicollis Meyer, 1931 with a description of Profilicollis rancoensis n. sp. (Acanthocephala: Polymorphidae) from the freshwater crab, Aegla abtao Schmitt, 1942 (Decapoda: Anomura) in Chile, with a key to congeneric species.

Profilicollis rancoensis n. sp. is the tenth species of Profilicollis Meyer, 1931 which includes 9 other species mostly known from marine decapod crabs and shore birds. Cystacanths of P. rancoensis are described from the dominant freshwater crab Aegla abtao in Ranco Lake, Chile and are morphologically distinguished from cystacanths of the 9 other species based on a combination of 4 characters. These are body size, number of proboscis hook rows, number of hooks per row, and length of the largest anterior 2-4 hooks. Male and female cystacanths of P. rancoensis are 2.10-3.33 mm long having an ovoid proboscis with 14 rows of 6-7 hooks per row, with the largest anterior 2-4 hooks being 105-110 micrometers long; the anterior trunk has many small spines in 70-80 concentric rings, each with 50-60 spines around them; hook roots are simple, directed posteriorly, about as long as the blades anteriorly with unremarkable anterior manubria; the cephalic ganglion are in mid-receptacle just anterior to the level of the anterior trunk; the lemnisci are long and slender; the testes are in the anterior trunk, posterior trunk, or one in each; the primordia of 2 tubular cement glands are evident; strong bundles of fibers link the anterior and posterior trunk; and the posterior trunk has a corrugated surface cuticula. Molecular analysis (COI and 18S) sequences coincided with the morphology and support its taxonomy. The phylogenetic profile revealed that P. rancoensis n. sp. fell into the Profilicollis clade. Both sequences showed low genetic variation, and three different haplotypes were found. The new species was more closely related to P. botulus (Van Cleave, 1916) Witenberg, 1932 than to other Profilicollis species.

Title: Révision du concept de Profilicollis Meyer, 1931 avec la description de Profilicollis rancoensis n. sp. (Acanthocephala, Polymorphidae) du crabe d'eau douce Aegla abtao Schmitt, 1942 (Decapoda, Anomura) au Chili, avec une clé des espèces congénères. Abstract: Profilicollis rancoensis n. sp. est la dixième espèce de Profilicollis Meyer, 1931 qui comprend neuf autres espèces principalement connues de crabes décapodes marins et d'oiseaux de rivage. Les cystacanthes de P. rancoensis sont décrits chez le crabe d'eau douce dominant Aegla abtao dans le lac Ranco, au Chili et se distinguent morphologiquement des cystacanthes des neuf autres espèces sur la base d'une combinaison de quatre caractères. Il s'agit de la taille du corps, du nombre de rangées de crochets du proboscis, du nombre de crochets par rangée et de la longueur des 2 à 4 crochets antérieurs les plus grands. Les cystacanthes mâles et femelles de P. rancoensis mesurent de 2,10 à 3,33 mm de long et ont une trompe ovoïde avec 14 rangées de 6 à 7 crochets par rangée, les 2 à 4 crochets antérieurs les plus grands mesurant 105 à 110 micromètres de long ; le tronc antérieur a de nombreuses petites épines en 70-80 anneaux concentriques chacun avec 50-60 épines ; les racines des crochets sont simples, dirigées vers l'arrière, à peu près aussi longues que les lames vers l'avant avec une manubrie antérieure sans particularité ; les ganglions céphaliques sont au milieu du réceptacle juste en avant du niveau du tronc antérieur ; les lemnisques sont longs et minces ; les testicules sont dans le tronc antérieur, le tronc postérieur ou un dans chacun ; les ébauches des 2 glandes cémentaires tubulaires sont évidentes ; de solides faisceaux de fibres relient le tronc antérieur et postérieur ; le tronc postérieur a une cuticule à surface ondulée. Les séquences d'analyse moléculaire (COI et 18S) coïncidaient avec la morphologie et confirmaient sa taxonomie. Le profil phylogénétique a révélé que P. rancoensis n. sp. appartient au clade Profilicollis. Les deux séquences ont montré une faible variation génétique et trois haplotypes différents ont été trouvés. La nouvelle espèce était plus proche de P. botulus (Van Cleave, 1916) Witenberg, 1932 que des autres espèces de Profilicollis.

Does experimental seaweed cultivation affect benthic communities and shorebirds? Applications for extensive aquaculture.

Extensive seaweed aquaculture is a growing industry expected to expand globally due to its relatively low impact and benefits in the form of ecosystem services. However, seaweeds are ecosystem engineers that may alter coastal environments by creating complex habitats on previously bare mudflats. These changes may scale up to top-consumers, particularly migratory shorebirds, species of conservation concern that regulate trophic webs at these habitats. Understanding how habitats are transformed and how this affects different species is critical to direct ecological applications for commercial seaweed management. We experimentally assessed through a Before-After Control-Impact design the potential changes exerted by Gracilaria chilensis farming on bare mudflats on the abundance, biomass, and assemblage structure of benthic macroinvertebrates, and their scaled-up effects on shorebirds' habitat use and prey consumption. As predicted, experimental cultivation of G. chilensis significantly affects different components of biodiversity that scale-up from lower to upper trophic levels. The total biomass of benthic macroinvertebrates increased with seaweed cultivation and remained high for at least 2 months after harvest, boosted by an increase in the median size of polychaetes, particularly Nereids. Tactile-foraging shorebirds tracked these changes at the patch level increasing their abundance and spending more time foraging at seaweed cultivated plots. These results suggest that seaweed farming has the potential to impact shorebird populations by favoring tactile-foraging species which could lead to a competitive disadvantage to species that rely on visual cues. Therefore, the establishment of new seaweed farms in bare mudflats at key sites for shorebirds must be planned warranting habitat heterogeneity (i.e., cultivated and non-cultivated areas) at the landscape level and based on a previous experimental approach to account for local characteristics. Fostering properly designed extensive seaweed farming over other aquaculture industries with greater negative environmental impacts would provide benefits for human well-being and for ecosystem functions.

Consumption of marine-derived nutrients from invasive Chinook salmon (Oncorhynchus tshawytscha) transfer ω-3 highly unsaturated fatty acids to invasive resident rainbow trout (O. mykiss).

Marine-derived nutrients (MDN) contained in gametes (mature eggs and sperm), carcasses and metabolic wastes from anadromous migratory salmon can transfer energy and materials to fresh water, thereby affecting the structure and function of stream ecosystems. This is crucial among ecosystems where humans have mediated biological invasions by propagating non-native species. Previous studies have demonstrated that consumption of MDN from salmon can benefit both native and invasive resident fishes. Yet, a more detailed understanding of the transfer of biomolecules with important physiological functions such as ω-3 highly unsaturated fatty acids (HUFAs) have received less attention among researchers. Here we demonstrate that consumption of MDN contained in invasive Chinook salmon eggs transfers ω-3 HUFAs (e.g., EPA and DHA) to resident invasive rainbow trout in a river food web. We conducted a field study in river sections previously identified as spawning areas for Chinook salmon in the Cisnes River, Patagonia. Rainbow trout were sampled around salmon spawning areas before, during, and after the salmon spawning season. Additionally, we collected tissue from different food web resources and components of different origin (e.g., primary producers, aquatic and terrestrial items) from the Cisnes River system. Analyses of stomach contents of trout were performed in conjunction with analyses of both lipid content and fatty acid profiles of trout tissue and food web components. Chinook salmon eggs showed higher content of ω-3 HUFAs, especially EPA (31.08 ± 23.08 mg g DW-1) and DHA (27.50 ± 14.11 mg g DW-1) than either freshwater or terrestrial components (0-6.10 mg g DW-1 both EPA and DHA). We detected marked shifts in the fatty acid profile (~six-fold increase in EPA and DHA) of trout following consumption of Chinook salmon eggs. Our findings suggest that MDN via consumption of salmon eggs by resident rainbow trout may positively influence resident trout and likely contribute to gauge synergistic interactions between invaders on receiving ecosystems of Patagonia.

Human Activity in Antarctica: Effects on Metallic Trace Elements (MTEs) in Plants and Soils.

Colobanthus quitensis (Kunt) is one of the two vascular plant species present in Antarctica and develops under severe environmental conditions, being found in both pristine and human-threatened environments. We determined the Cd, Cr, Cu, Mn, Ni, Pb, and Zn levels in C. quitensis roots, leaves, and soils of origin using flame atomic absorption spectroscopy. In January 2017, we collected samples from four geographical zones on the longitudinal gradient along which C. quitensis is distributed, starting from Punta Arenas (PAR) at the extreme south of mainland Chile and moving southwards to the Antarctic territory from King George Island (KGI) to Hannah Point Peninsula (PHA) and finally Lagotellerie Island (LAT). We used certified reference material to validate the plant tissues and soil samples we collected. The highest concentrations of metals that we measured in the soils and in the C. quitensis roots and leaves were in samples we collected at the KGI station, the zone with the greatest human activity. The lowest concentrations we measured were at the LAT station, an island with little human intervention and scarce fauna. The mean concentrations of metals in the roots and leaves of C. quitensis followed a similar order at all sampling locations: Mn > Zn > Cu > Ni > Pb > Cr > Cd. In contrast, in soil, they followed the following order: Mn > Zn > Cu > Cr > Pb > Ni > Cd. The concentration levels obtained for the different metals in the soil and plants tissue samples in this region of Antarctica indicated that the area was non-polluted. However, the metallic trace element (MTE) concentrations may be at an early stage of contamination, as described in other areas of the Antarctic, being a new threat to this continent.

Temporal Variability of Macroinvertebrate Assemblages in a Mediterranean Coastal Stream: Implications for Bioassessment.

Macroinvertebrates from a small forest stream in central-south Chile were sampled monthly from September 2017 to August 2018 to assess temporal variability in the assemblage and the effects of that variability on ecological indicators. Higher precipitation and flows occurred in winter months, and water quality varied among months. We collected 59 macroinvertebrate taxa, finding higher taxa richness and abundances in summer months than in winter months. Four taxa demonstrated marked seasonality, being abundant in some months and then decreasing in following months (Limnoperla jaffueli (Navás), Nousia maculata (Demoulin), Smicridea sp. (McLachlan), Chironomidae spp.). The scores of the family Hilsenhoff Biotic Index (HBI), Hilsenhoff Species-level Biotic Index (HSBI), Multimetric Macroinvertebrate Index (MMI), and Chilean Biological Monitoring Working Party (ChBMWP) index varied throughout the year reflecting natural variability. However, only HBI and HSBI scores were significantly different among seasons, ranging across three water quality classes (excellent, very good, and good), showing the lowest water classes in spring, coinciding with higher abundances of tolerant species. The MMI and ChBMWP indicated good and very good site conditions throughout the year, respectively. Shannon-Weaver diversity ranged between 2.59 (April) and 1.78 (February); however, Pielou evenness had high values throughout the year (> 0.62), except in February. Changes in macroinvertebrates composition throughout the year were explained primarily by discharge, water temperature, and conductivity. Our findings indicate that natural monthly variability in macroinvertebrate assemblages influences the scores of biological indices throughout the year. Therefore, we recommend that natural stream variability be accounted for in biomonitoring programs. We also emphasize the need to use caution when interpreting biological index scores to avoid misinterpretations in stream quality classification.

Interplay of geomorphology and hydrology drives macroinvertebrate assemblage responses to hydropeaking.

Hydropeaking leads to major anthropogenic disturbance of river networks worldwide. Flow variations imposed by hydropeaking may significantly affect macroinvertebrate assemblages within the river network. As such, the responses of macroinvertebrate assemblages to hydropeaking are expected to be complex and vary across spatial and temporal scales as well as ecological organization levels. To unpack this complexity, we assessed the interplay of geomorphic and hydrological variables as drivers of the responses of macroinvertebrate assemblages to hydropeaking. Specifically, we studied different levels of ecological organization of macroinvertebrate assemblages in two functional process zones (FPZs; Sub-Andean and Central Valley Gravel Dominated) subjected to different flow management in two Chilean Andean river networks. Hydropeaking caused significant reduction of macroinvertebrate abundances in both FPZs and at all ecological organization levels with the exception of one feeding guild (scrapers). Furthermore, the response of macroinvertebrate assemblage variance was stronger in the Central Valley Gravel Dominated FPZ. Both geomorphic and hydrological variables influenced macroinvertebrate assemblage responses. However, the effects of the principal geomorphic variables operated at valley (meso) spatial scale and the main hydrological variables operated at the sub-daily (micro) temporal scale. Therefore, to minimise the effects of hydropeaking on macroinvertebrate assemblages, flow management should consider reduction of sub-daily variability. Furthermore, placement of new barriers should take into account not only their position within the river network but also their effects downstream that strongly depend on characteristics of river valley.

A Century after! Rediscovery of the ancient catfish Diplomystes Bleeker 1858 (Siluriformes: Diplomystidae) in coastal river basins of Chile and its implications for conservation

The ancient catfish family Diplomystidae, with seven species endemic to rivers of southern South America, represents one of the oldest branches of the diverse order Siluriformes. With most species endangered, new reports of these species become extremely valuable for conservation. Currently, it is assumed that Diplomystes species inhabit only Andean (large) basins, and that they are extinct from coastal (small) basins from which their presence have not been recorded since 1919. Here, we document new records of the family Diplomystidae in the Laraquete and Carampangue basins, two coastal basins from the Nahuelbuta Coast Range, Chile, with no previous reports. This finding represents the rediscovery of the genus in coastal basins in more than a Century. Based on analysis of mitochondrial DNA sequences, the collected specimens were found to be closely related to Diplomystes nahuelbutaensis from the Andean Biobío Basin, but sufficiently differentiated to suggest that coastal basin populations are a different management unit. These populations are important because, contrary to previous thoughts, they prove these catfish can survive in small river networks, providing unique opportunities for research and conservation. The conservation category of Critically Endangered (CE) is recommended for the populations from the Laraquete and Carampangue basins.(AU)

La familia de bagres Diplomystidae, con siete especies endémicas de ríos del sur de Sudamérica, es uno de los linajes mas antiguos del diverso orden Siluriformes. Al estar la mayoría de las especies amenazadas, nuevos registros de éstas son extremadamente valiosos para su conservación. Actualmente, se ha asumido que los Diplomystidos se distribuyen solo en cuencas Andinas (más grandes), y que sus especies estarían extintas en cuencas de menor tamaño como las costeras, sin registros desde 1919. En este trabajo documentamos la familia Diplomistidae en las cuencas de Carampangue y Laraquete, dos cuencas costeras de la Cordillera de Nahuelbuta, Chile, lo que representa el primer registro de esta familia en estas cuencas costeras. Además, este hallazgo representa el re-descubrimiento de la familia en cuencas costeras después de un siglo. Sobre la base de análisis de ADN mitocondrial, los especímenes colectados se relacionaron más cercanamente con poblaciones de la especie Diplomystes nahuelbutaensis presente en la cuenca del Biobío. Sin embargo, existen diferencias genéticas suficientes entre las poblaciones costeras y las del Biobío para justificar su separación como unidad de manejo distinta. Estas poblaciones costeras son importantes porque demuestran que los Diplomístidos pueden sobrevivir en cuencas de pequeño tamaño, ofreciendo oportunidades únicas para su investigación y conservación. Se recomienda la categoría de conservación En Peligro Critico de Extinción (CR) para las poblaciones de las cuencas Laraquete y Carampangue.(AU)

[A case report of hand, foot, and mouth disease with necrotizing mucocutaneous lesions]. / Reporte de caso de enfermedad mano-pie-boca con lesiones mucocutáneas que evolucionan a necrosis.

In most cases, the cause of hand, foot, and mouth disease (HFMD) is coxsackievirus A type 16. The infection can also be caused by other strains of coxsackievirus, spreading mainly by the oral-fecal route, while it is less likely to be transmitted through secretions. HFMD occurs mainly in summer and is more common in children under ten. Skin lesions develop during the disease but rarely become necrotic. When present, they are a severe complication requiring hospitalization. This paper reports the case of a patient with HFMD who developed necrotic mucocutaneous lesions that responded favorably to intravenous acyclovir, fluids, and electrolyte support therapy.

La enfermedad de mano-pie-boca es una patología originada en la mayoría de los casos por el virus coxsackie A tipo 16, aunque también puede ser ocasionada por otras cepas de la familia de los coxsackievirus. Dicho virus se propaga principalmente por vía fecal oral y, en menor proporción, por secreciones. Se presenta principalmente en verano, siendo frecuente en niños menores de 10 años. Dentro de dicha enfermedad las lesiones mucocutáneas que evolucionen en necrosis son poco frecuentes, constituyéndose en una complicación severa que requiere hospitalización. En el presente artículo se reporta un caso con diagnóstico de enfermedad mano-pie-boca, que evolucionó hacia lesiones mucocutáneas necróticas, mostrando una respuesta favorable a una terapia de soporte de aciclovir, líquidos y electrolitos.

Examining the influence of human stressors on benthic algae, macroinvertebrate, and fish assemblages in Mediterranean streams of Chile.

Changes in land-uses and cover are strong drivers of stream degradation worldwide. In Mediterranean ecoregions, land conversion from forest with native species to tree plantations, agricultural, and urban areas have drastically increased in recent years. Here, we examined the influence of land-use types on water quality (physical and chemical variables) and biota (benthic algae, macroinvertebrates, and fishes). We conducted field surveys between December-March 2016 (dry season) in 24 sites located across four land-use types including native vegetation (NV), tree plantation (TP), agriculture (Ag), and urban (Ur) within the Chilean Mediterranean ecoregion. We calculated metrics and multivariate statistics to assess correlations between biological and environmental variables. Significant differences between land-uses were found for environmental/physicochemical variables and the three biotic groups (i.e., benthic algae, macroinvertebrates, and fishes). We found higher chlorophyll-a biomass for TP, Ag, and Ur compared to NV streams. The macroinvertebrate diversity of sensitive taxa (i.e., Ephemeroptera, Plecoptera, and Trichoptera) and water quality were lower in land draining streams with human uses, other than NV, with the most evident impact found in Ag and Ur streams. NV and TP streams had a lower Diptera density and lower percentage of non-insect taxa. Fish richness and diversity were not affected by land-use. However, cold-water fishes (both native and introduced) were closely associated with NV and TP streams whereas warm-water fishes (mostly introduced) were closely associated with Ag and Ur streams. Multivariate analysis revealed that both local habitat and catchment-scale variables influenced each biological assemblage. Our findings suggest that benthic algae, macroinvertebrates, and fish populations are good indicators of human land-use disturbances, with Ag and Ur areas being the most detrimental for freshwater biota.

Tumores infrecuentes en pediatría: tumor de ovario: caso clínico / Infrequent tumors in pediatrics: ovarian tumor presentation of a case and bibliographic review

Introducción: Los tumores malignos de ovario son raros en la edad pediátrica, con una incidencia de 1-2 por cada 1 000 000 habitantes, caracterizados por dolor abdominal y efecto de masa, cuya edad de diagnóstica varia teniendo dos picos de incidencia entre 8 a 9 años de vida y entre los 13 a 17 años. Objetivo: Identificar los puntos critico que influyeron en el diagnóstico y tratamiento de niña con tumor de ovario. Descripción del caso: Referimos el caso de una adolescente de 14 años que ingresa al servicio de pediatría, por presentar dolor abdominal, distención abdominal y vómito. Al ser evaluada se encuentra una gran distención abdominal con una masa dura que abarca especialmente hemi-abdomen derecho. La TAC y ECO revelan masa quística dependiente de ovario derecho que desplaza asas intestinales, además la presencia de derrame pleural bilateral que lleva al diagnóstico de metástasis pulmonar. No se realiza examen histopatológico debido a que la paciente acude a recibir atención médica especializada en estadios avanzados de la enfermedad al proceder de área rural. Conclusiones: Se describe un caso de niña a la que se le realiza diagnóstico de tumor de ovario avanzado y metástasis pulmonar, que no se realiza examen histopatológico por provenir de área rural, recibir tratamientos naturales por periodo prolongado lo que impidieron realizar diagnóstico y tratamiento oportuno

Introduction: Malignant ovarian tumors are rare in the pediatric age, with an incidence of 1-2 per 1 000 000 persons. Introduction: Malignant ovarian tumors are rare in the pediatric age, with an incidence of 1-2 per 1 000 000 inhabitants, characterized by abdominal pain and mass effect, whose diagnostic age varies with two peaks of incidence between 8 to 9 years of life and between 13 to 17 years. Objetive: To identify the critical points that influenced the diagnosis and treatment of a girl with an ovarian tumor. Case description: We report the case of a 14-year-old girl who enters the pediatric service, due to abdominal pain, abdominal distention and vomiting. When evaluated, a large abdominal distension is found with a hard mass that especially includes the right hemi-abdomen. CT and ECO reveal cystic mass dependent on the right ovary that displaces intestinal loops, in addition to the presence of bilateral pleural effusion that leads to the diagnosis of pulmonary metastasis. Histopathological examination is not performed because the patient goes to receive specialized medical attention in advanced stages of the disease when coming from rural area. Conclusions: We describe a case of a girl who is diagnosed with advanced ovarian tumor and pulmonary metastasis, who did not undergo histopathological examination due to being from rural areas, receiving natural treatments for a prolonged period, which prevented diagnosis and timely treatment

Effects of local land-use on riparian vegetation, water quality, and the functional organization of macroinvertebrate assemblages.

Land-use change is a principal factor affecting riparian vegetation and river biodiversity. In Chile, land-use change has drastically intensified over the last decade, with native forests converted to exotic forest plantations and agricultural land. However, the effects thereof on aquatic ecosystems are not well understood. Closing this knowledge gap first requires understanding how human perturbations affect riparian and stream biota. Identified biological indicators could then be applied to determine the health of fluvial ecosystems. Therefore, this study investigated the effects of land-use change on the health of riparian and aquatic ecosystems by assessing riparian vegetation, water quality, benthic macroinvertebrate assemblages, and functional feeding groups. Twenty-one sites in catchment areas with different land-uses (i.e. pristine forests, native forests, exotic forest plantations, and agricultural land) were selected and sampled during the 2010 to 2012 dry seasons. Riparian vegetation quality was highest in pristine forests. Per the modified Macroinvertebrate Family Biotic Index for Chilean species, the best conditions existed in native forests and the worst in agricultural catchments. Water quality and macroinvertebrate assemblages significantly varied across land-use areas, with forest plantations and agricultural land having high nutrient concentrations, conductivity, suspended solids, and apparent color. Macroinvertebrate assemblage diversity was lowest for agricultural and exotic forest plantation catchments, with notable non-insect representation. Collector-gatherers were the most abundant functional feeding group, suggesting importance independent of land-use. Land-use areas showed no significant differences in functional feeding groups. In conclusion, anthropogenic land-use changes were detectable through riparian quality, water quality, and macroinvertebrate assemblages, but not through functional feeding groups. These data, particularly the riparian vegetation and macroinvertebrate assemblage parameters, could be applied towards the conservation and management of riparian ecosystems through land-use change studies.

Resecciones esternales. Técnica utilizada / Sternal resections. Applied technique

Se presentan dos casos de tumores primitivos de esternón que fueron tratados con amplias resecciones. Los defectos parietales ubicados uno en el tercio superior y otro en el tercio inferior, fueron reparados con un "sandwich" de Marlex y metil metacrilato, con una cobertura de epiplón, permitiendo con esta combinación obtener un buen resultado anatómico, cosmético y funcional brindando también una razonable protección contra infecciones